Figure 3.

Reversible Oxidation Levels of Cysteine Residues in Aging D. melanogaster

(A) Lifespan of control female flies. Cohorts of flies were taken to analyze protein cysteine residue redox state of young (7 days), middle-aged (28 days), and old (56 days) flies.

(B) Distribution of cysteine peptides plotted against their redox states for 7-, 28-, and 56-day-old control flies. Data are means ± SEM. The red curve is for 7-day-old control flies.

(C) Oxidation state of cysteine residues present in 56-day-old flies plotted against 7-day-old flies. The dotted line slope = 1, while the continuous line is the least-squares best-fit line to the data. Data from 263 unique peptides identified at least three times under both conditions are plotted. Red symbols (n = 6) indicate low-stringency significance with p < 0.05 assessed by a non-paired, two-tailed Student’s t test.

See also Figure S3.