Fig. 2.

Kinase inhibitors of Src-selective autophagy. (A) FAK −/− cells were treated with the inhibitors A–F for 24 h. Cells were fixed and stained with anti-p-Src Y416 (green), anti-paxillin (red) and DAPI (blue). Scale bars: 20 μm. (B) Percentage of cells with active Src localising to intracellular structures was quantified and data presented as mean ± s.d. Significance for all treatments is p < 0.01 (n = 3). (C) LC3B was immunoprecipitated from FAK −/− cell lysates treated with kinase inhibitors. Then immunoblotting was performed using anti-p-Src Y416 and anti-LC3B antibodies.