Table 1.

A–F inhibitors in this study from Tocriscreen Kinase Inhibitor Toolbox. SCC cells were plated overnight then the inhibitors from the Tocriscreen Kinase Inhibitor Toolbox were added at 1 μM, 10 μM or 20 μM for 24 h. Plates were fixed and stained with anti-p-Src Y416, Deep Red Cell Mask and DAPI, then imaged using a Scan-R microscope. Scan-R analysis software was used to calculate the number of puncta (based on size and shape of Src positive puncta) per cell (calculated using DAPI) and to identify any alterations to the intracellular localisation of active Src. The effect that each inhibitor had on active Src is summarised in this table.

Inhibitor	Inhibitor name	Target	20 μM	10 μM	1 μM
A	SB218078	Chk1	Less p-Src Y416 puncta	Less p-Src Y416 puncta	Less p-Src Y416 puncta
B	PD407824	Chk1	Toxic for cells	Cells visibly unhealthy	Less p-Src Y416 puncta
C	SB216763	GSK-3β	Less p-Src Y416 puncta	Less p-Src Y416 puncta	No detectable change
D	BIO	GSK-3β	Toxic for cells	Less p-Src Y416 puncta	Less p-Src Y416 puncta
E	ZM306416	VEGFR	Less p-Src Y416 puncta	No detectable change	No detectable change
F	Ki8751	VEGFR	Less p-Src Y416 puncta	Less p-Src Y416 puncta	Less p-Src Y416 puncta

Note: The above named inhibitors, designated A–F, were used as agents to inhibit Src-selective autophagy in the absence of FAK. We think it is likely that these may work in this context by off target effects.