Figure 1.

Morphological and phenotypic characteristics of BM-MSCs. The cells isolated from rat bone marrow exhibited spindle shaped fibroblast-like morphology. (a) Primary culture, passage zero (P0), (b) P1, (c) P2, and (d) P3. (Phase contrast microscope, scale bar = 100 µm.) (e) A representative flow cytometric analysis of cell-surface markers of BM-MSCs at P3. Cells were labeled with antibodies against MSC marker (CD44-PE) and hematopoietic antigen (CD45-FITC); M1 is the % of cells positive for both markers.