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HRM analysis failed to detect relatively high levels of A750G heteroplasmy but was able to detect low levels of G750A heteroplasmy at C. felis cytb nucleotide 750 in clinical samples. The red cluster is anticipated to have only adenine at nucleotide 750, the green cluster is anticipated to have only guanine at nucleotide 750, and the orange and blue clusters are anticipated to have heteroplasmy at nucleotide 750. RFU, relative fluorescence units.
