TABLE 4.

Positive predictive value for identification of C. felis cytb1 is highest when all five nucleotide positions in PCR panel are analyzed

cytb nucleotide(s) analyzed	Specificity of cytb1 detection (%)	Positive predictive value (%)
		With cytb1 population prevalence of 8%	With cytb1 population prevalence of 18.8%	With cytb1 population prevalence of 26.7%
750A	75	25.8	48.1	59.3
750A + 947T	85.7	37.8	61.8	71.8
750A + 947T + 399A	91.1	49.4	72.2	80.4
750A + 947T + 399A + 274C	96.4	70.7	86.5	91
750A + 947T + 399A + 274C + 858G	98.2	82.9	92.8	95.3