TABLE 1.
MALDI-TOF identification of E. coli flagella representing the most common serotypesa
| Strain no. (H type) | MS-H typeb | Sequence coveragec (%) for test: |
||
|---|---|---|---|---|
| 1 | 2 | 3 | ||
| EDL-933 (H7) | H7 | 71 | 66 | 68 |
| 09-1767 (H11) | H11 | 57 | 64 | 56 |
| 85-489 (H21) | H21 | 78 | 87 | 86 |
| E32511 (nonmotile) | No flagellin detected | NA | NA | NA |
a
The three most common E. coli strains and one nonmotile strain were independently cultured and analyzed by MS-H with DHB as the MALDI matrix on three separate occasions. Results were obtained by a Mascot database search.
b
The top hit resulting from mass profiling of at least three of four sample-loaded grid spots.
c
The average sequence coverage of MALDI grid spots (representing sample repeats) that gave correct identification. NA, not applicable.