TABLE 1.

DNA concentration and reference genome coverage for DNA extracted from directly lysed glycerol stocks or from recultured aliquots^a

Year	Sample no.	DNA from directly lysed samplesb					DNA from recultured samples					Comparison
		Qubit (HS) before purification (ng/μl)	Qubit (HS) after purification (ng/μl)	Cov any (mean)	Cov any (%)	Cov unamb (%)	Visible growth	Qubit (HS) after 1:2 dilution (ng/μl)	Cov any (mean)	Cov any (%)	Cov unamb (%)	Library from (gly/LJ)	No. of SNPs
1992	GE0005	0.08	0.07	52.1	99.4	98.3	No					Yes/no
1992	GE0006	0.22	0.16	63.6	99.4	98.2	Yes	29	92.4	99.4	98.5	Yes/yes	0
1992	GE0007	0.39	0.15				Yes	79	56.4	99.2	97.6	No/yes
1992	GE0008	0.71	0.37	61.7	99.4	98.3	Yes	16	83.7	99.5	98.7	Yes/yes	0
1992	GE0009	0.71	0.23	64.7	99.3	98.3	No					Yes/no
1992	GE0011	0.47	0.99				No					No/no
1992	GE0012	0.18	0.18	94.3	98.9	98.0	No					Yes/no
2002	GE0022	Too low	0.53	64.4	99.4	98.1	Yes	63	111.3	99.4	98.6	Yes/yes	0
2003	GE0028	0.37	0.37	59.9	99.2	97.7	Yes	87	90.0	99.3	98.2	Yes/yes	0
2003	GE0030	0.30	0.75	92.4	99.4	98.5	Yes	57	78.7	99.4	98.5	Yes/yes	0
2005	GE0039	0.20	0.17	68.0	99.2	97.9	Yes	74	111.6	99.4	98.6	Yes/yes	0
2005	GE0040	0.70	0.22	92.3	99.5	98.9	Yes	37	118.4	99.4	98.6	Yes/yes	0
2005	GE0041	0.40	0.27	70.8	99.4	98.3	Yes	33	105.3	99.5	98.7	Yes/yes	0
2005	GE0042	0.71	0.91	63.6	99.3	97.6	Yes	14	107.1	99.4	98.7	Yes/yes	0
2005	GE0044	0.48	0.30	71.0	99.3	97.9	Yes	42	102.2	99.4	98.5	Yes/yes	0
2005	GE0045	0.09	1.30	73.4	99.3	98.1	Yes	50	89.3	99.3	98.3	Yes/yes	0
2005	GE0046	0.36	0.34	66.5	99.3	98.1	Yes	90	89.5	99.4	98.6	Yes/yes	0
2005	GE0047	0.76	0.89	67.7	99.3	98.2	Yes	28	77.2	99.4	98.5	Yes/yes	0
2005	GE0048	0.32	0.44	54.4	99.3	98.0	Yes	37	65.1	99.3	98.2	Yes/yes	0
2005	GE0049	0.29	0.24	59.3	99.3	98.0	Yes	42	58.2	99.4	98.2	Yes/yes	0
2005	GE0050	0.36	0.39	64.2	99.3	97.8	Yes	26	56.0	99.4	98.3	Yes/yes	0
2006	GE0052	0.22	0.78	71.7	99.3	98.1	Yes	37	102.5	99.5	98.8	Yes/yes	0
2005	GE0054	1.93	2.32	73.0	99.3	98.0	Yes	50	87.1	99.4	98.7	Yes/yes	0
2005	GE0055	1.53	3.17	75.3	99.4	98.0	Yes	20	65.3	99.4	98.4	Yes/yes	0
2006	GE0056	0.79	1.21	82.5	99.4	98.3	Yes	59	85.9	99.4	98.6	Yes/yes	0
2006	GE0057	0.69	0.90	56.3	99.4	97.9	Yes	81	93.0	99.3	98.5	Yes/yes	0
2006	GE0058	0.66	0.64	67.6	99.3	97.9	Yes	34	75.3	99.4	98.4	Yes/yes	0
2008	GE0069	0.68	0.68	67.6	99.4	98.1	Yes	65	91.6	99.4	98.6	Yes/yes	0
2008	GE0070	0.30	0.37	69.8	99.4	98.1	Yes	20	67.9	99.3	98.2	Yes/yes	0
2009	GE0072	1.05	1.18	55.7	99.2	97.6	Yes	74	85.0	99.4	98.5	Yes/yes	0
2009	GE0073	1.28	1.47	87.0	99.2	97.6	Yes	31	69.3	99.4	98.5	Yes/yes	0
2009	GE0074	1.56	1.61	70.2	99.2	97.5	Yes	101	111.3	99.5	98.8	Yes/yes	0
2009	GE0077	2.05	3.76	75.7	99.3	98.1	Yes	71	88.5	99.4	98.6	Yes/yes	0
2009	GE0078	2.15	2.55	69.3	99.4	98.1	Yes	107	118.4	99.5	98.8	Yes/yes	1
2009	GE0079	2.01	1.45	59.4	99.3	97.9	Yes	169	117.1	99.5	98.9	Yes/yes	1
2009	GE0080	0.66	0.54	60.3	99.1	96.9	Yes	65	72.3	99.3	98.2	Yes/yes	0
2009	GE0081	1.87	1.66	64.7	99.3	97.8	Yes	50	60.9	99.3	98.1	Yes/yes	0
2009	GE0082	1.44	1.43	63.0	99.2	97.6	Yes	98	75.1	99.4	98.4	Yes/yes	0
2012	GE0148	0.69	0.70	70.2	98.8	97.7	Yes	33	56.3	98.8	97.5	Yes/yes	0
2012	GE0151	0.70	0.65	67.9	99.3	97.9	Yes	91	112.2	99.5	98.8	Yes/yes	0

^aIn total, analysis of sequencing data from paired DNA samples differed in two cases. An additional SNP was found for the recultured aliquot: for sample GE0078 one SNP at position 1471870 in the rRNA gene Rvnr01 and for sample GE0079 one nonsynonymous SNP at position 3199069 in the Rv3085 gene.

^bHS, high sensitivity; cov, genome coverage; unamb, unambiguous; gly, glycerol stocks.