TABLE 3.

Identification of genetic markers of resistance in monomicrobial cultures using BC-GN assay

Target	Specimen type	No. with resulta:				Total no.	% (95% CI) forb:
		TP	FP	TN	FN		PPA	NPA
blaCTX-M	Fresh	45	0	657	1c	703	97.8 (87–100)	100.0 (99–100)
	Frozen	28	0	733	1c	762	96.6 (80–100)	100.0 (99–100)
	Simulated	108	0	226	0	334	100.0 (96–100)	100.0 (98–100)
	Total	181	0	1,616	2	1,799	98.9 (96–100)	100.0 (99–100)
blaKPC	Fresh	2	0	701	0	703	100.0 (20–100)	100.0 (99–100)
	Frozen	1	0	761	0	762	100.0 (5–100)	100.0 (99–100)
	Simulated	50	0	284	0	334	100.0 (91–100)	100.0 (98–100)
	Total	53	0	1,746	0	1,799	100.0 (92–100)	100.0 (99–100)
blaNDM	Fresh	1	0	702	0	703	100.0 (5–100)	100.0 (99–100)
	Frozen	0	0	762	0	762	ND	100.0 (99–100)
	Simulated	50	0	282	2d	334	96.2 (86–100)	100.0 (98–100)
	Total	51	0	1,746	2	1,799	96.2 (86–100)	100.0 (99–100)
blaOXA	Fresh	6	0	696	1e	703	85.7 (42–99)	100.0 (99–100)
	Frozen	3	0	757	2e	762	60.0 (17–93)	100.0 (99–100)
	Simulated	57	1f	275	1e	334	98.3 (90–100)	99.6 (98–100)
	Total	66	1	1,728	4	1,799	94.3 (85–98)	99.9 (99–100)
blaVIM	Fresh	0	0	703	0	703	ND	100.0 (99–100)
	Frozen	0	0	762	0	762	ND	100.0 (99–100)
	Simulated	68	0	266	0	334	100.0 (93–100)	100.0 (98–100)
	Total	68	0	1,731	0	1,799	100.0 (93–100)	100.0 (99–100)
blaIMP	Fresh	0	0	703	0	703	ND	100.0 (99–100)
	Frozen	0	0	762	0	762	ND	100.0 (99–100)
	Simulated	47	0	287	0	334	100.0 (91–100)	100.0 (98–100)
	Total	47	0	1,752	0	1,799	100.0 (91–100)	100.0% (99–100)

^aTP, true positive, FP, false positive, TN, true negative, FN, false negative.

^bPPA, positive percent agreement; NPA, negative percent agreement; 95% CI, 95% confidence interval; ND, not detected.

^cConfirmed to be bla_CTX-M-1 by sequence analysis.

^dOne of two confirmed to be bla_NDM by sequence analysis. One was not available for discrepant analysis.

^eConfirmed to be bla_OXA-23 by sequence analysis.

^fNegative for bla_OXA by sequence analysis.