TABLE 4.
Identification of Gram-negative organisms in polymicrobial cultures using BC-GN assay
| Parameter | Results for: |
||
|---|---|---|---|
| Reference culture | BC-GN | Final calla | |
| Two organisms (1 and 2), both on BC-GN panel | (1) K. pneumoniae, (2) E. asburiae | (1) K. pneumoniae, (2) Enterobacter sp. | (1) TP, (2) TP |
| (1) K. pneumoniae, (2) A. lwoffii | (1) K. pneumoniae, (2) Acinetobacter sp. | (1) TP, (2) TP | |
| (1) K. pneumoniae, (2) P. aeruginosa | (1) K. pneumoniae, (2) NDc | (1) TP, (2) FNb | |
| (1) K. pneumoniae, (2) P. aeruginosa | (1) K. pneumoniae, (2) ND | (1) TP, (2) FNb | |
| (1) K. oxytoca, (2) E. cloacae | (1) K. oxytoca, (2) ND | (1) TP, (2) FNd | |
| (1) E. coli, (2) K. oxytoca | (1) E. coli, (2) K. oxytoca | (1) TP, (2) TP | |
| (1) E. coli, (2) K. oxytoca | (1) E. coli, (2) ND | (1) TP, (2) FNe | |
| (1) E. coli, (2) K. oxytoca | (1) E. coli, (2) ND | (1) TP, (2) FNe | |
| (1) E. coli, (2) P. mirabilis | (1) E. coli, (2) Proteus sp. | (1) TP, (2) TP | |
| (1) E. coli, (2) P. mirabilis | (1) E. coli, (2) Proteus sp. | (1) TP, (2) TP | |
| (1) E. coli, (2) K. pneumoniae | (1) E. coli, (2) ND | (1) TP, (2) FNf | |
| (1) E. coli, (2) K. pneumoniae | (1) E. coli, (2) ND | (1) TP, (2) FNf | |
| (1) E. coli, (2) K. pneumoniae | (1) E. coli, (2) K. pneumoniae | (1) TP, (2) TP | |
| (1) E. coli, (2) K. pneumoniae | (1) E. coli, (2) K. pneumoniae | (1) TP, (2) TP | |
| (1) E. coli, (2) P. aeruginosa | (1) E. coli, (2) ND | (1) TP, (2) FNb | |
| (1) E. coli, (2) A. baumannii | (1) E. coli, (2) Acinetobacter sp. | (1) TP, (2) TP | |
| (1) E. coli, (2) E. aerogenes | (1) E. coli, (2) Enterobacter sp. | (1) TP, (2) TP | |
| (1) E. coli, (2) E. cloacae | (1) ND, (2) ND | (1) FN, (2) FNg | |
| (1) E. cloacae, (2) K. oxytoca | (1) Enterobacter sp., (2) K. oxytoca | (1) TP, (2) TP | |
| (1) E. cloacae, (2) K. pneumoniae | (1) Enterobacter sp., (2) ND | (1) TP, (2) FNg | |
| (1) E. cloacae, (2) K. pneumoniae | (1) Enterobacter sp., (2) K. pneumoniae | (1) TP, (2) TP | |
| (1) E. cloacae, (2) A. baumannii | (1) Acinetobacter sp., (2) Enterobacter sp. | (1) TP, (2) TP | |
| Two organisms (1 and 2), with one of two on BC-GN panel | |||
| (1) E. coli, (2) E. faecium | (1) E. coli, (2) ND | (1) TP, (2) TN | |
| (1) E. coli, (2) E. faecalis | (1) E. coli, (2) ND | (1) TP, (2) TN | |
| (1) E. coli, (2) Aeromonas sp. | (1) E. coli, (2) ND | (1) TP, (2) TN | |
| (1) E. coli, (2) Aeromonas sp. | (1) E. coli, (2) ND | (1) TP, (2) TN | |
| (1) K. oxytoca, (2) S. marcescens | (1) K. oxytoca, (2) ND | (1) TP, (2) TN | |
| (1) K. oxytoca, (2) E. hermannii | (1) K. oxytoca, (2) Citrobacter sp. | (1) TP, (2) FPg | |
| (1) P. aeruginosa, (2) Kocuria sp. | (1) P. aeruginosa, (2) ND | (1) TP, (2) TN | |
| (1) P. aeruginosa, (2) Sphingomonas sp. | (1) P. aeruginosa, (2) ND | (1) TP, (2) TN | |
| (1) E. cloacae, (2) Morganella sp. | (1) Enterobacter sp., (2) ND | (1) TP, (2) TN | |
| (1) E. cloacae, (2) E. faecium | (1) Enterobacter sp., (2) ND | (1) TP, (2) TN | |
| (1) E. cloacae, (2) Hafnia sp. | (1) Enterobacter sp., (2) ND | (1) TP, (2) TN | |
| (1) A. baumannii, (2) S. maltophilia | (1) Acinetobacter sp., (2) ND | (1) TP, (2) TN | |
| (1) Acinetobacter radioresistens, (2) CoNSh | (1) Acinetobacter sp., (2) ND | (1) TP, (2) TN | |
| Three organisms | |||
| (1) E. coli, (2) K. pneumoniae, (3) A. baumannii | (1) E. coli, (2) K. pneumoniae, (3) A. baumannii | (1) TP, (2) TP, (3) TP | |
| (1) E. coli, (2) P. mirabilis, (3) Sphingomonas sp. | (1) E. coli, (2) P. mirabilis, (3) ND | (1) TP, (2) TP, (3) TN | |
| (1) K. pneumoniae, (2) K. oxytoca, (3) Enterobacter sp. | (1) K. pneumoniae, (2) K. oxytoca, (3) ND | (1) TP, (2) TP, (3) FNi | |
| (1) K. oxytoca, (2) Pseudomonas stutzeri, (3) S. maltophilia | (1) K. oxytoca, (2) ND, (3) ND | (1) TP, (2) TN, (3) TN | |
| (1) K. oxytoca, (2) P. stutzeri, (3) S. maltophilia | (1) K. oxytoca, (2) ND, (3) ND | (1) TP, (2) TN, (3) TN | |
a
Final call versus reference culture result. TP, true positive; TN, true negative; FP, false positive; FN, false negative.
b
P. aeruginosa confirmed by sequence analysis.
c
ND, not detected.
d
Identification of the organisms in this specimen could not be resolved by sequence analysis.
e
K. oxytoca confirmed by sequence analysis.
f
K. pneumoniae confirmed by sequence analysis.
g
Specimens (15131, 17155, and 17111) were not available for sequence analysis.
h
CoNS, coagulase-negative Staphylococcus species.
i
Sequence analysis failed to identify K. oxytoca in the specimen.