. 2015 Jul 20;53(8):2768–2772. doi: 10.1128/JCM.00647-15

TABLE 3.

Serum and cerebrospinal fluid antibody and nucleic acid testing in eastern equine encephalitis^a

Parameter^b	Value(s) or result for patient:
Parameter^b	1	2	3	4	5	6	7	8
Serum
EEEV antibody hemagglutination inhibition titer(s)	1:320, 1:1,280, and 1:5,270^c^,^d	1:40^e	ND	ND	ND	ND	ND	ND
EEEV antibody neutralization titer	ND	1:80^e	ND	ND	ND	ND	ND	ND
EEEV immunoglobulin M antibody immunofluorescence titer	ND	ND	ND	ND	ND	ND	≥1:256^f	ND
EEEV immunoglobulin G antibody immunofluorescence titer	ND	ND	<1:16^f	1:16^f	ND	1:32^f	1:512^f	≥1:256^g
WNV immunoglobulin M antibody enzyme-linked immunoassay titer	ND	ND	ND	ND	−^f	ND	−^f	ND
WNV immunoglobulin G antibody enzyme-linked immunoassay titer	ND	ND	ND	ND	ND	−^f	−^f	ND
Specimen obtained on hospital day	5, 8, 12^c	2	1	2	2	1	6	61^g
Cerebrospinal fluid
EEEV immunoglobulin M antibody	ND	ND	ND	1:32 ^f	ND	ND	<1:1^h^,ⁱ, 1:1^j^,^f	ND
EEEV immunoglobulin G antibody	ND	ND	ND	ND	ND	ND	1:1^j^,^f	ND
EEEV PCR	ND	ND	+^k	−^k	+^k	+^k	+^j^,^k	+^k
WNV immunoglobulin M antibody	ND	ND	−^k	ND	ND	ND	−^j^,^k	ND
WNV immunoglobulin G antibody	ND	ND	+^k	ND	ND	ND	ND	ND
WNV PCR	ND	ND	−^k	−^k	−^k	−^k	−^j^,^k	−^k
HSV PCR	ND	ND	−^k	−^k	−^k	−^k	−^j^,^k	−^k
Specimen obtained on hospital day(s)	1	1	1	2	2	1	1^h, 6^j	1

+, positive; −, negative; ND, not done.

EEEV, eastern equine encephalitis virus; WNV, West Nile virus; HSV, herpes simplex virus.

Specimens were obtained on hospital days 5, 8, and 12, respectively.

Testing for antibody was performed at the Virus Laboratory, Department of Health of the State of New York, Albany, NY.

Testing for antibody was performed at the Centers for Disease Control, Atlanta, GA.

Testing for antibody was performed at the Diagnostic Immunology Laboratory, Department of Health of the State of New York, Albany, NY.

Specimen was obtained on day 61 after admission to hospital, when the patient was at home after discharge from the hospital.

Specimen was obtained on hospital day 1.

ⁱ

Testing for antibody was performed by Laboratory Corporation of America, Raritan, NJ.

Specimen was obtained on hospital day 6.

Testing for nucleic acid was performed at the Viral Encephalitis Testing Laboratory, Department of Health of the State of New York, Albany, NY, using a commercially available nucleic acid method, as previously published (Dupuis et al. [8]; Huang et al. [9]; Hull et al. [10]).