FIGURE 2.

Blue light using flavin adenine dinucleotide (BLUF) domain-regulated adenylate cyclases. (A) The euPACα polypeptide is composed of two BLUF and two catalytic domains in the order BLUF1, C1, BLUF2, C2, and likely dimerizes or tetramerizes when expressed heterologously. The C1 and C2 catalytic domains associate to form the adenylate cyclase active site. BLUF domains N-terminal to each catalytic domain enhance catalysis in response to light. (B) The bPAC is composed of a single BLUF and a single catalytic domain, and likely dimerizes when expressed, so that an adenylate cyclase active site forms at the interface of the catalytic domains. The BLUF domain enhances catalysis in response to light.