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. 2015 Jul 21;8:37. doi: 10.3389/fnmol.2015.00037

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Copyright © 2015 Zhou, Pan and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recent uses of LOV and CRY2 domains with potential neuroscience applications. (A) Motor protein direction can be modified by changing the lever arm length using a LOV2 domain. An artificial lever arm was made by fusing two α-actinin structural elements (arm and extension) to LOV2, then this was attached to a motor protein’s catalytic domain (motor body). Change in lever arm geometry upon illumination resulted in a change in motor direction. (B) Light-induced LOV2 conformational change was also used to disrupt the folding of two fragments of calmodulin, allowing light-induced release of calcium. (C) The LARIAT method enables temporary inactivation of a POI by sequestration. LARIAT consists of CRY2 conjugated to a POI or an antibody fragment recognizing the POI (Ab) and CIB1 conjugated to a multimerizing protein (M). Blue light excitation causes the POI to aggregate to the multimer clusters formed by the CIB1-M construct.