Figure 5.
(A,B) Clubroot symptoms and (C), quantification of Plasmodiophora brassicae DNA in infected roots of the partially resistant accession Bur-0 and the susceptible accession Col-0. (A) Clubroot symptoms were evaluated using the GA/LA disease index calculated by image analysis at 17 and 21 dpi. GA/LA was calculated from gall area (GA in cm2) divided by an estimation of the rosette extent (LA in cm2). Error bars represent standard error (Four biological replicate, 18 plants analyzed per biological replicate). Asterisks indicate statistically significant differences according to the (P < 0.05) (B) Illustration of clubroot symptoms. The scale bar indicates 1 cm. (C) Pathogen DNA quantification (Pb) by qPCR, expressed as a ratio relative to the expression level of the plant Fbox gene at 10, 14, and 17 dpi (Three biological replicates, 12–54 plants per biological replicate). Asterisks indicate statistically significant differences according to the Wald tests applied on a linear mixed model (P < 0.05).