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. 2015 Jul 21;5:12229. doi: 10.1038/srep12229

Figure 8. Selection of optimal TFP and production of endopolygalacturonase (KmEPG1) by using the mini-translational fusion partner (TFP) library.

Figure 8

(a) SDS-PAGE analysis of KmEPG1 expressed by selected TFPs. A 0.6-mL aliquot of the culture supernatant was analysed on a 10% Tricine gel after precipitation with acetone. M: standard protein size marker, lane 1: TFP1-4, lane 2: TFP2, lane 3: TFP3, lane 4: TFP3-1, lane 5: TFP18, lane 6: TFP16, lane 7: TFP4, lane 8: TFP5, lane 9: TFP6, lane 10: TFP7-1, lane 11: TFP8, lane 12: TFP9, lane 13: TFP10, lane 14: TFP11, lane 15: TFP12, lane 16: TFP13, lane 17: TFP14-1, lane 18: TFP15, lane 19: TFP16-3, lane 20: TFP17-3, lane 21: TFP18-1, lane 22: TFP19-1, lane 23: TFP20, lane 24: TFP14. (b) Profile of fed-batch fermentation and SDS-PAGE analysis of fermentation broth (10 μL) retrieved at the indicated times. (c) Comparison of KmEPG1 secretion level between native secretion signal (lane 1) and TFP3 (lane 2). The protein was revealed by Coomassie staining.