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Chromatographic purification of S. japonicum paramyosin. A pET-30 plasmid containing paramyosin was expressed and purified similarly as described (28). Lane 1, inclusion body preparation; lane 2, anion exchange chromatography; lane 3, size exclusion chromatography; lane 4, purified S. japonicum paramyosin with a thioredoxin fusion tag. Reprinted from Ref. (30).
