Table 3. TA-cloning and Sanger sequencing results for mulberry miRNAs identified in silkworm tissues.
| Fat body (C/T) | Silk gland (C/T) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| miRNA name | 1st sequencing | 2nd sequencing | 1st sequencing | 2nd sequencing | Brain (C/T) | Prothoracic gland (C/T) | Salivary gland (C/T) | Gut (C/T) | Malpighian tubule (C/T) | Ovary (C/T) | Testis (C/T) |
| mno-miR169a | 2.63% | 0.00% | 0.00% | 0.00% | 0.00% | 2.17% | 0.00% | 0.00% | 0.00% | 2.33% | 0.00% |
| mno-miR166b | 20.55% | 76.19% | 56.76% | 59.46% | 53.66% | 31.71% | 9.09% | 8.57% | 10.81% | 25.00% | 13.33% |
| mno-miR166c | 54.05% | 95.45% | 72.22% | 93.02% | 59.52% | 61.76% | 20.83% | 24.39% | 42.11% | 31.82% | 37.50% |
| mno-miR167e | 12.50% | 2.27% | 13.51% | 2.33% | 25.64% | 9.76% | 6.67% | 8.11% | 20.00% | 7.32% | 8.16% |
| mno-miR396b | 73.17% | 65.96% | 55.81% | 45.45% | 87.23% | 66.67% | 36.84% | 31.71% | 48.78% | 34.09% | 22.44% |
| mno-miR159a | 0.00% | – | 5.00% | – | – | – | – | – | – | – | – |
| mno-miR156c | 8.33% | – | 2.17% | – | – | – | – | – | – | – | – |
| mno-miR398 | 0.00% | – | 0.00% | – | – | – | – | – | – | – | – |
| mno-miR162 | 0.00% | – | 0.00% | – | – | – | – | – | – | – | – |
Results are shown for one control mulberry miRNA (mno-miR169a) and four mulberry miRNAs (mno-miR166b, mno-miR166c, mno-miR167e and mno-miR396b) in silkworm tissues (brain, prothoracic gland, salivary gland, gut, malpighian tubule, ovary, testis, fat body, and silk gland). The TA-cloning of the five miRNAs (mno-miR169a, mno-miR166b, mno-miR166c, mno-miR167e, and mno-miR396b) was performed twice in fat body and silk gland, once in other tissues. The percentage represents the sequencing frequency of correct clones relative to the total clones. “–” indicates that we did not clone the miRNA in the fat body, silk gland, brain, prothoracic gland, salivary gland, gut, malpighian tubule, ovary, or testis.