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. 2015 Jul 21;5:12292. doi: 10.1038/srep12292

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Copyright © 2015, Macmillan Publishers Limited

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qPCR validation of the 18 putative targets of miR-15a, miR-15b and miR-16 detected as significantly downregulated by Affymetrix arrays in hyperosmotically perfused livers. Downregulation of Aatf, Bfar, and Ikbkb may contribute to the promotion of a proapoptotic phenotype. Statistical analysis was carried out by unpaired student’s t-test. Data are shown as average ± S.E.M. of 4 independent experiments. qPCR runs were normalized according to the ΔΔCt method using β-Tubulin as reference gene. The values of unstimulated controls were set arbitrarily to 100.