Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 21;5:12292. doi: 10.1038/srep12292

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

mRNA analysis of target genes of the miR-15 family under hyper- and normoosmotic conditions in perfused rat liver. mRNA levels of hyper- and normoosmotically treated samples at specific time points (30, 60, 120 and 180 minutes) were compared to unstimulated controls (T0). (a,b) Bcl2 and Ccnd1 are significantly downregulated after 120 and 180 minutes of hyperosmotic perfusion. (c) Mcl1 is upregulated under normoosmotic conditions, while this effect is attenuated under hyperosmotic conditions. (d) The anti-apoptotic molecule Faim is significantly downregulated under hyperosmotic conditions and stably expressed in the normoosmotic control. qPCR runs were normalized according to the ΔΔCt method using Gapdh as reference gene. Statistical analysis was carried out by unpaired student’s t-test. Data are shown as average ± S.E.M. of 5 independent experiments. The values of unstimulated controls (T0) were set arbitrarily to 100.