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. 2015 Jul 21;5:12292. doi: 10.1038/srep12292

Figure 5. Benzylamine upregulates miR-15a/16 in a NAC-sensitive manner.

Figure 5

Rat livers were perfused with benzylamine (0.2 mmol/l) and glucose (5 mmol/l). In the control, NAC was added with a concentration of 10 mmol/l. (a) miR-15a is significantly upregulated after 60 (p = 0.0031), 120 (p = 0.0184) and 180 minutes (p = 0.0062) of benzylamine perfusion. miR-16 is significantly upregulated after 60 minutes of benzylamine perfusion (p = 0.0245). (b) The target gene Bcl2 is significantly downregulated after 30 (p = 0.0421) and 180 minutes (p = 0.0131) of benzylamine perfusion, while Faim is significantly downregulated after 180 minutes of benzylamine perfusion (p = 0.022). (c) In presence of NAC the benzylamine-induced activation of miRNA was inhibited. (d) Bcl2, Cyclin D1 and Mcl1 are unchanged in presence of NAC, while Faim is significantly downregulated after 180 minutes of perfusion (p = 0.0468). qPCR runs were normalized according to the ΔΔCt method using β-tubulin as reference gene. Statistical analysis was carried out by unpaired student’s t-test. Data are shown as average ± S.E.M. of 3 independent experiments. The values of unstimulated controls (T0) were set arbitrarily to 100.