Figure 6. Upregulation of miR-15a/b and miR-16 by hyperosmolarity in perfused rat liver is blocked by administration of SP600125.

(a–d) Addition of the JNK inhibitor SP600125 does not lead to significant changes of miRNAs under both hyperosmotic and normoosmotic conditions. qPCR runs were normalized according to the ΔΔCt method using RNU6 as reference gene. Statistical analysis was carried out by unpaired student’s t-test. Data are shown as average ± S.E.M. of 3 independent experiments. The values of unstimulated controls (T0) were set arbitrarily to 100.