Table 1. Description of the miR-15b and miR-16 putative targets downregulated under hyperosmotic conditions.

Gene Name	Description	Seed Length	Seed Sequence	Binding Start	Binding End	mRNA Region
Aatf	Apoptosis antagonizing transcription factor	7	AGCAGCA	1751	1745	3 UTR
Bfar	Bifunctional apoptosis regulator	8	AGCAGCAC	1606	1599	3 UTR
C3ar1	Complement component 3a receptor 1	7	UAGCAGC	1924	1918	3 UTR
Dcbld2	Discoidin, CUB and LCCL domain containing 2	8	AGCAGCAC	2334	2327	3 UTR
Gstm3	Glutathione S-transferase mu 3	7	AGCAGCA	1160	1154	3 UTR
Ikbkb	Inhibitor of kappa light polypeptide: enhancer in B-cell	9	UAGCAGCAC	2584	2576	3 UTR
Irak2	Interleukin-1 receptor-associated kinase 2	8	UAGCAGCA	2628	2621	3 UTR
Itgal	Integrin, alpha L	7	UAGCAGC	3960	3954	3 UTR
Leprot	Leptin receptor overlapping transcript	8	UAGCAGCA	1646	1639	3 UTR
Nanp	N-acetylneuraminic acid phosphatase	7	AGCAGCA	915	909	3 UTR
Nras	Neuroblastoma RAS viral oncogene homolog	7	AGCAGCA	771	765	3 UTR
Oas1b	2′-5′-oligoadenylate synthetase 1	8	UAGCAGCA	1776	1769	3 UTR
Sema4a	Sema domain, immunoglobulin domain	7	AGCAGCA	2781	2775	3 UTR
Slc11a2	Solute carrier family 11, member 2	8	UAGCAGCA	1998	1991	3 UTR
Slc16a6	Solute carrier family 16 member 6	7	AGCAGCA	1939	1933	3 UTR
Tbp	TATA box binding protein	9	UAGCAGCAC	1440	1432	3 UTR
Them4	Thioesterase superfamily member 4	8	AGCAGCAC	1342	1335	3 UTR
Zfp105	Zinc finger protein 105	8	UAGCAGCA	1861	1854	3 UTR

Analysis of both bioinformatics (miRWalk) and gene chip data led to 18 potential target genes of the miR-15a, miR-15b and miR-16.

Predicted binding sites within the 3′UTR of the potential target genes are listed. Among them, several genes are involved in apoptotic processes, such as Aatf, Bfar or Ikbkb.