Fig. 1.

Microtubule-associated protein (MAP) 2e and von Willebrand factor (vWF) immunohistochemical staining of human GBM samples. A: MAP2e, a splice variant of MAP2, was a candidate glioma-specific antigen. Tumor cells diffusely infiltrated from the tumor center to normal brain tissue; there is no border between them. B: At the tumor border, MAP2e-positive tumor cells clustered around dilated vessels. C: Single MAP2e-positive tumor cell infiltration into normal brain parenchyma that are independent of vasculature were also seen. MAP2e: diaminobenzidine (DAB), vWf: DAB-Ni, Counterstain: hematoxylin.