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. 2015 Jul 22;16(1):540. doi: 10.1186/s12864-015-1748-1

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© Gupta et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Comparison of expression of 37 selected genes based on the analysis of the Illumina sequencing data using Cuffdiff and DESeq and the corresponding qRT-PCR data. The heat map visualises the expression of 37 regulated immune genes 12 h after pricking of larvae and workers with a 1:1 mix of Gram-negative and Gram-positive bacteria in case of Illumina sequencing (Cuffdifflog2FC / DeSeq log2FC). The corresponding qRT-PCR analysis was performed separately in larvae and workers revealing a stage-specific gene regulation after immune challenge (log2(x-fold change L2 + W2)). Up and down-regulation are colour coded as given in the key highlighting common directions of gene regulation in the different data sets