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. 2015 Jul 21;5:12360. doi: 10.1038/srep12360

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Copyright © 2015, Macmillan Publishers Limited

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(a) Overexpression of PCBP4 protein in IMC-3PCBP4 cells was confirmed by Western blotting. GAPDH was used as a loading control. The quantified density of the blots is presented in the right panel. (b) The cell cycle population was observed using flow cytometry without stimulation. *P < 0.05. Error bars ± s.d. (c) The cell cycle population was analyzed using flow cytometry after cisplatin treatment (1 μg/ml, 48 h). *P < 0.05. Error bars ± s.d. (d) Apoptotic cells were confirmed using Annexin V after cisplatin treatment (1 μg/ml, 48 h). *P < 0.01. Error bars ± s.d. (e) Cell viability analyzed by MTT assay after cisplatin treatment (1 μg/ml, 48 h). *P < 0.05. Error bars ± s.d.