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. 2015 Jul 21;5:12360. doi: 10.1038/srep12360

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(a) RNA immunoprecipitation and RT-PCR. Quality control total RNA means whole RNA before immunoprecipitation (lane 3). IP signifies immunoprecipitation by anti-PCBP4 specific antibody (lane 1) and normal IgG as a negative control (lane 2). GAPDH is shown as a housekeeping gene. (b) Expression of Cdc25A mRNA was analyzed using quantitative real-time PCR after cisplatin treatment (1 μg/ml, 6 h). *P < 0.05. Error bars ± s.d. (c) Protein levels of Cdc25A, Cdc25B, and Cdc25C were analyzed by Western blot after cisplatin treatment (1 μg/ml, 24 h). GAPDH is used as a loading control.