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. 2015 Jul 20;210(2):347–361. doi: 10.1083/jcb.201501018

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© 2015 Prospéri et al.

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Figure 6. — Myo1b and fascin are required for HCT116 cell repulsion. (A) YFP-EphB2-HCT116 cells transfected with control, Myo1b, or fascin siRNAs were cocultivated with Cherry-ephrinB1-HCT116. Representative sequences of merged YFP and Cherry fluorescent focal plane at the base of the cells illustrating the behavior of YFP-EphB2-HCT116 cells that contact Cherry-ephrinB1-HCT116 and correspond to Video 5 are shown. Bars, 15 µm. The yellow boxes mark the regions shown in the insets and enlarged by 1.4. The yellow lines on the merged images at time 0 represent the region of the kymographs shown in B. Filopodia with concentration of EphB2 at their tips are formed only in cells transfected with control siRNA. (B) Kymographs at the interface of EphB2 and the ephrinB1-expressing cells from Video 5. In the absence of Myo1b or fascin, EphB2 cells remain in contact with ephrinB1 cells.