Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 20;210(2):303–318. doi: 10.1083/jcb.201411001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Gomez-Lamarca et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Aberrant trafficking of Notch, Dl, and Wg in Rme-8–depleted cells. (A–B″) Effects of Rme-8 Ri on Notch (anti-NICD, red in A and B; anti-NECD, green in B) and Dl (green in A) trafficking. Both, full-length Notch and Dl accumulate together inside Rme-8-Ri cells (arrowheads). Depleted territory in this and subsequent figures is on the right side of dashed line, unless stated otherwise. (C and C′) NECD (staining without detergent) is depleted from the membrane when Rme-8 is knocked down (Rme-8^KK). (D) Quantification of surface NECD levels from C. The data shown are from a single representative disc; n = 6 for the experiment shown; the experiment was repeated three times with similar results. Line shows mean fluorescence at the membrane level within the specified compartment. (E and E′) Intracellular Notch puncta accumulate in Rme-8^C19 mutant cells (GFP negative, marked by dashed line; generated as in Fig 1 I). (F and F′) Wg is detected more broadly in the Rme-8-Ri tissue.