Figure 2.

miR-182 regulated NRN1 expression and altered cell proliferation. (A) Northern blotting demonstrated a difference in the hybridization signal. The human U6 probe was used as a loading control. (B) and (C) reverse transcription-quantitative polymerase chain reaction analysis, and (D) and (E) western blotting analysis of neuritin mRNA and protein expression, respectively, within the different groups. (F) A CCK8 assay was used to determine cell survival. *P<0.05 and ^#P>0.05 vs. mutant miR-182-transfected cells. n=3. miR, microRNA; NRN1, neuritin 1.