Effects of electroacupuncture on the expression levels of various protein involved in the Wnt/β-catenin signaling pathway. (A) Representative western blot, where β-actin was used as the internal control. Quantitative determination of the protein expression levels of (B) LRP5, (C) β-catenin and (D) Runx2 in the femurs of the different groups, as determined by western blot analysis. Data are presented as the mean ± standard deviation. *P<0.01, vs. sham group; #P<0.01, vs. OVX group. LRP5, low-density lipoprotein receptor-related protein 5; Runx2, runt-related transcription factor 2; OVX, ovariectomized; GV, governor vessel; BL, bladder meridian.