Table II.
Sequences, base pair lengths, annealing temperatures and elongation times of the primers used.
| Gene | Sequence, 5-3 | Base pairs | Annealing, °C/sec | Elongation, °C/sec | |||
|---|---|---|---|---|---|---|---|
| β-actin | F: CCAACCGCGAGAAGATGA | 97 | 65/3 | 68/4 | |||
| R: CCAGAGGCGTACAGGGATAG | |||||||
| MAGE-A1 | F: GGCCGAAGGAACCTGACC | 69 | 67/3 | 72/4 | |||
| R: GTCCTCTGGGTTGGCCTGT | |||||||
| MAGE-A5 | F: GCCCTAGAGGAGCACCAAAG | 80 | 62/4 | 72/3 | |||
| R: CGCAACAGGCAGGAGTGT | |||||||
| MAGE-A8 | F: AAAGGTTCGCAGAGAACAGG | 119 | 65/3 | 72/3 | |||
| R: GTCAGGGCAGCAGGAGAGT | |||||||
| MAGE-A9 | F: GGCCTTGGTCTGAGACAGTG | 97 | 65/3 | 72/3 | |||
| R: GTCCTCCTGGTTAGCCTGT | |||||||
| MAGE-A11 | F: ACAGGAGTCCCAGGAGAACC | 81 | 67/3 | 72/4 | |||
| R: CTGTGGGAAATATCTGGGTGA | |||||||
| MAGE-A12 | F: GTCGGTGGAGGGAAGCAG | 104 | 65/3 | 72/3 | |||
| R: AGGGCAGCAGGTAGGAGTG |
MAGE, melanoma-associated antigen; F, forward; R, reverse.