Figure 2.

Assessment of MIF mRNA expression by reverse transcription-polymerase chain reaction analysis in endometrial samples. A 399-bp product of MIF mRNA was visualized with ethidium bromide following agarose gel electrophoresis. GAPDH (452 bp) was used as a control to assess the amount of RNA in each sample. (A and B) Proliferative endometrium from (A) women with endometriosis and (B) controls. (C and D) Secretory endometrium from (C) women with endometriosis and (D) controls. Quantification of the MIF mRNA level was performed using internal GAPDH as a reference. Results are presented as the mean ± standard deviation. ^aP<0.05, compared with the normalized density of MIF mRNA in the secretory endometrial tissues of the controls. MIF, macrophage migration inhibitory factor.