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. 2015 Jun 11;10(2):709–715. doi: 10.3892/ol.2015.3371

Figure 5.

Figure 5.

Piperlongumine (PPLGM) promotes c-Jun phosphorylation at Ser63. Protein expression levels were determined by western blotting. β-actin was used to verify equal loading. (A) HCT116 cells were exposed to PPLGM at increasing concentrations for 48h. (B) HCT116 cells were treated with 10 µM PPLGM for the indicated times. Figures were selected as representative data from three independent experiments. p-, phosphorylated; t-, total.