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. 2015 Jun 2;10(2):1117–1122. doi: 10.3892/ol.2015.3310

Figure 4.

Figure 4.

Effect of jolkinolide B on protein expressions of the β1-integrin/FAK and ERK signaling proteins. (A and B) Effect of jolkinolide B on the expression of the proteins of the β1-integrin/FAK signaling pathway. Cells were treated with 0, 1 and 2 µM jolkinolide B. (A) Proteins were extracted, and the expression of β1-integrin, FAK and p-FAK was then determined by western blot analysis. (B) Densitometric values of protein bands were normalized to those of GAPDH. The data represent the mean ± SD of three independent determinations. *P<0.05 vs. untreated cells. (C and D) The effect of jolkinolide B on the expression of ERK signaling proteins. (C) Expression of the ERK signaling proteins in MDA-MB-231 cells was confirmed by western blot analysis. (D) Quantitated results are presented relative to the control. Densitometric analysis of western blotting was obtained from three independent experiments and is expressed as the mean ± SD. *P<0.05 vs. untreated cells. SD, standard deviation; FAK, focal adhesion kinase; p-FAK, phosphorylated FAK; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; p-Akt, phosphorylated Akt.