Figure 4.

Treatment with SchB induces cell cycle arrest at G0/G1 phase. A549 cells were treated with, or without, different concentrations of Sch B for 72 hours, fixed, and treated with RNase I and PI. The distribution of different phases of cells was determined by flow cytometry. Data are representative histograms, images and expressed as the means ± SD of individual groups of cells from three independent experiments. A. Flow cytometry analysis. B. Quantitative analysis. C. Western blot analysis. D. Quantitative analysis of the relative levels of target protein to the GAPDH. *P < 0.05 vs. the control, determined by ANOVA and post hoc Bonferroni multiple comparisons test.