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. 2015 May 15;8(5):7049–7058.

Figure 2.

Figure 2

Transfection of Beclin 1 for BT-549 and MDA-MB-231 cells. A. BT-549 and MDA-MB-231 cells were tranfected with pDS-RED-C1-Beclin 1 or pDsRed-C1 plasmid, cells were harvested and analyzed by western blot and qPCR. B. Morphology of BT-549 and MDA-MD-231 cells post transfection of pDS-RED-C1-Beclin 1 plasmid were observed under fluorescence microscopy. C. Morphology of BT-549 and MDA-MD-231 cells post transfection of pDS-RED-C1-Beclin 1 or pDsRed-C1 plasmid were observed under fluorescence microscopy, staining by acridine orange. *P<0.05 vs. blank group. The results are representative of three different experiments.