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. 2015 May 15;8(5):7535–7543.

Figure 3.

Figure 3

Epigenetic modifications in L-PK. A. Methylation level of the L-PK promoter. B. L-PK promoter activity assay in vitro. C. ChREBP-L-PK binding in vivo using a ChIP assay. D. Histone H3 acetylation status around the L-PK gene. E. Histone H3K9 acetylation status around the L-PK gene. F. Histone H3K18 acetylation status around the L-PK gene. G. Histone H4 acetylation status around the L-PK gene. H. Histone H4K8 acetylation status around the L-PK gene. I. Histone H4K12 acetylation status around the L-PK gene. J. Histone H3K9me3 methylation status around the L-PK gene. n≥4. *P<0.05; **P<0.01 vs. Ctrl or PGL3-basic. #P<0.05; ##P<0.01 vs. HFD or L-PK (Sss-). Ctrl, control group; HFD, high-fat diet-induced NAFLD group; HFD+BBR, Berberine treatment group; pGL3-basic, transfected with plasmid pGL3-Basic; L-PK (Sss-), transfected with the pGL3-L-PK promoter (unmethylated); L-PK (Sss+), transfected with the pGL3-L-PK promoter (methylated).