Figure 4.

hPOT1-RNAi-induced apoptosis and senescence. (A) Terminal deoxynucleotidyl transferase dUTP nick end labeling assay of Hep-2R cells after expression with pshRNA-hPOT1. The apoptotic cells in the Hep-2R, control and RNAi groups were determined after 0, 12, 24 and 48 h. (B) Growth curves of Hep-2R cells expressing pshRNA-hPOT1 and vector control cells. The difference in proliferative capacity between the Hep-2R and RNAi groups was significant. RNAi, RNA interference; pshRNA, plasmid short hairpin RNA; hPOT1, human protection of telomere 1.