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. 2015 Jun 4;10(2):717–722. doi: 10.3892/etm.2015.2546

Figure 4.

Figure 4.

HIF-1α is a direct target of miR-18a. (A) Predicted duplex formation between the 3′-UTR of HIF-1α and miR-18a. (B) Luciferase activity following the incorporation of the wild-type (UTR-WT) or mutant (UTR-mut) HIF-1α 3′-UTR reporter gene in the MGC-803 cells transfected with miR-18a or NC mimics. (C) Quantitative polymerase chain reaction analysis showing the mRNA expression levels of HIF-1α in the MGC-803 and HGC-27 cell lines transfected with the miR-18a or NC mimics. Data were normalized against the mRNA expression level of GAPDH. (D) Western blot analysis showing the protein expression levels of HIF-1α in the MGC-803 and HGC-27 cell lines transfected with the miR-18a or NC mimics. *P<0.05. HIF, hypoxia-inducible factor; UTR, untranslated region; miR, microRNA; NC, negative control.