Figure 1.

Mitophagy induced by amino acid starvation and CCCP treatment in SH-SY5Y cells is blocked by lysosomal inhibition. SH-SY5Y cells were treated with CCCP (A) or EBSS (B) for the indicated times and also treated with the lysosomal inhibitor HCQ 3 h before analysis to block lysosomal degradation. MTDR was used to determine mitochondrial staining by flow cytometry. * Indicates comparisons between control (Co) and treatments (CCCP and EBSS). ^# Indicates differences between presence and absence of HCQ within treatments. * or ^#P < 0.05, ** or ^##P < 0.01, *** or ^###P < 0.001 (C) Western blot analysis for the indicated mitochondrial proteins in SH-SY5Y cells treated as above for the indicated times.