Figure 3.
Nicotinamide and fisetin induce mitophagy in a lysosomal-dependent manner, an effect blocked by different lysosomal inhibitors. SH-SY5Y cells were incubated with NAM, FIS, PHEN, or CCCP for 6 h and mitophagy determined (A) after treatment with the lysosomal inhibitor HCQ 3 h before analysis, or in the continued presence of (B) bafilomycin A1 (BafA1) or (C) ammonium chloride+leupeptin (N+L). * Indicates comparisons between control (Co) and treatments. # Indicates differences between presence and absence of HCQ, BafA1 or N+L within treatments. * or #P < 0.05, ** or ##P < 0.01, *** or ###P < 0.001