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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Int J Med Microbiol. 2005 Jan;294(7):447–453. doi: 10.1016/j.ijmm.2004.09.010

Fig. 3.

Fig. 3

Stability of Tn4001mod insertions in M. agalactiae and M. bovis as revealed by Southern hybridization using a Tn4001-specific probe. Genomic DNA of independent pISM2062 transformants was harvested after 10 or 20 additional passages in SP-4 broth, in presence (+) or absence (−) of Gm. (A) BamHI/EcoRI-digested DNA of non-transformed M. agalactiae (Ma) and its two different pISM2062 transformants, A1 and A2 (the same as depicted in lanes 1 and 5 of Fig. 2A, respectively). (B) BamHI/EcoRI-digested DNA of non-transformed M. bovis (Mb) and its pISM2062 transformant B1 (the same as depicted in lane 14 of Fig. 2B). Digested pISM2062 (P) containing the Tn4001mod served as a positive control in both cases.