Figure 2.

Example of Pseudomonas aeruginosa-specific high-titer phage-production protocol. Target strains of P. aeruginosa are incubated separately for 24 hours in the early exponential phase (OD 600 nm =0.1) with a known phage cocktail at a 0.035 multiplicity of infection (= volume of phage suspension/volume of bacterial suspension). After 24 hours, the obtained lysates are centrifuged at 5,000× g for 30 minutes at 4°C. Supernatant is filtered through a 0.2 μm membrane and stored at 4°C.

Abbreviations: CFU, colony-forming units; OD, optical density.