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. 2015 Jun 29;10(6):e0131614. doi: 10.1371/journal.pone.0131614

Fig 4. Effect of Grb2 and DDX3 on viral growth.

Fig 4

Human foreskin fibroblasts were treated with siRNA against the viral immediate early genes UL122/123 (IE1/2, positive control), the cellular gene Grb2, the cellular gene DDX3 or a non-targeting siRNA (negative control). Two days after siRNA treatment, cells were infected with HCMV strain TB40/E at an MOI of 1(A) or 0.01 (B). A: The titer of infectious progeny virus in cell free supernatants of cells infected at an MOI of 1 was determined at 5 d postinfection. Each matched pair of values represents an independent experiment. Knockdown of both Grb2 and DDX3 reduced virus progeny production. B: Focal growth in cultures infected at an MOI of 0.01 was determined by staining cells for the viral immediate early antigens and counting the number of foci per well and the number of infected cells per focus (focus size). The total effect on focal growth is represented by the product of focus number and focus size.