Table 1. The details for nested and standard PCR design and number of successful PCR reactions in vagina and stool samples.

		No. of successful PCR reactions
PCR name	PCR design	vagina	stool
nested PCRs
Nested_10–30	10 cycles by primer pair 1, then use the products for 30 cycles by primer pair 2	17	6
Nested_20–20	20 cycles by primer pair 1, then use the products for 20 cycles by primer pair 2	16	12
standard PCRs
Standard_10–30	10 cycles by primer pair 2, then use the products for 30 cycles by primer pair 2	17	11
Standard_20–20	10 cycles by primer pair 2, then use the products for 30 cycles by primer pair 2	17	12
Standard_40	40 cycles by primer pair 2	12	12

Note, Primer pair 1 is 515F and 1492R; primer pair 2 is the tagged 515F and 806R.