Fig 3. Size of induration after injection of L. infantum stationary phase promastigotes in 0.4% phenol-saline to measure skin test reactivity.

Data represent the individual delayed type of hypersensitivity responses in millimeter at months11 (A) and 16 (B) after infection. DTH reaction was determined by injecting intradermally in the inner aspect of the right hind leg with 0.1 ml of L. infantum in 0.4% phenol-saline antigen (3×10⁸ stationary phase promastigotes/ml). The left hind leg received only 0.1 ml 0.4% phenol saline solution. Data represent the increase of intradermal reaction performed 48 hrs after antigen injection. The values of the saline control were subtracted from the reaction due to the Leishmania antigen. Reactions ≥5mm were considered as positive.