Table 2. Vaccination regimens in different dog groups.
The first group (G1) was immunized subcutaneously (sc) with 2x107 L. tarA2-CPA-CPB-CTE GFP (recombinant (r) rLive/rLive). The second group (G2) was immunized sc with L. tarentolae (WT) and the third group (G3) was injected with PBS. Three weeks after priming, all groups were boosted similarly. Three weeks after the boost, all groups were challenged by intravenous (iv) injection of 4 x 107 L. infantum metacyclic promastigotes. PBS: Phosphate saline buffer.
| Groups | Priming (day 0) | Boosting (day 21) | Challenge (day 42) | Modality |
|---|---|---|---|---|
| Group 1 (N = 10) | L. tarentolaeA2-CPA-CPB-CTE-EGFP (sc) | L. tarentolaeA2-CPA-CPB-CTE-EGFP (sc) | L. infantum (iv) | rLive/rLive |
| Group 2 (N = 10) | L. tarentolae (WT) (sc) | L. tarentolae(WT) (sc) | L. infantum (iv) | rLive/rLive |
| Group 3(N = 10) | PBS (sc) | PBS (sc) | L. infantum (iv) | Control |