Table 3. Clinical status of dogs and percentage of each group within each category.

	Bone marrow PCR a	Spleen culture a	Clinical biochemistry abnormality	Weight lost	G1	G2	G3
Sub patent	+	-	None	None	34%	22%	11%
Asymptomatic	+	-	+b	Minorc	33%	44%	33%
Symptomatic	+	+	++b	Intensived	33%	34%	56%

^a L. infantum was detected by PCR in the bone marrow aspirates or by the culture of spleen collected 18 and 20 months post-infection, respectively.

+^b = lower than 3 biochemical parameters with abnormalities

++^b = more than 3 biochemical parameters with abnormalities

Minor^c = weight loss less than 2 Kg

Intensive^d = weight loss more than 3 Kg