Table 3.
Matrix for triple mixture composition
| Mixture | 200 gene Copies/reaction | 20 000 gene copies/reaction | 200 000 gene copies/reaction |
|---|---|---|---|
| 1 | A | B | C |
| 2 | A | B | D |
| 3 | A | C | D |
| 4 | A | D | C |
| 5 | A | C | B |
| 6 | A | D | B |
| 7 | B | A | C |
| 8 | B | A | D |
| 9 | B | C | D |
| 10 | B | D | C |
| 11 | B | C | A |
| 12 | B | D | A |
| 13 | C | A | B |
| 14 | C | A | D |
| 15 | C | B | D |
| 16 | C | D | B |
| 17 | C | B | A |
| 18 | C | D | A |
| 19 | D | A | B |
| 20 | D | A | C |
| 21 | D | B | C |
| 22 | D | C | B |
| 23 | D | B | A |
| 24 | D | C | A |
For multiplex real-time PCR (mRT-PCR) 1: A = Streptococcus pneumoniae; B = Haemophilus influenzae; C = Staphylococcus aureus; D = Moraxella catarrhalis.
For mRT-PCR 2: A = Escherichia coli; B = Klebsiella pneumoniae; C = Pseudomonas aeruginosa; D = Acinetobacter baumannii.