Fig. 2.

Testing 3D PLA printed device for spine morphology: (A) Hippocampal neuronal culture on PLA 3D-printed coverslip (Model 001, Model 005, Model 007). Neurons were transfected with Myr-EGFP and imaged at 21 d.i.v showing a healthy culture with spine densities and structurally indistinguishable from neurons transfected with the same vector and grown on commercial coverslips. Scale Bar: 10 μm. (B) ROI, Structure of spines at 63× in live culture. (C) 3D reconstruction of the ROI, showing the tridimensionality of spine architecture.